Tác giả: Nguyen Hoang Xuan Thao; 吳宗遠 博士 (người hướng dẫn).

中原大學 . Năm: 2015.

Mô tả: 60Tr, kích thước: 30cm. Số định danh: . Vị trí: Phòng đọc.

Tóm tắt:

Baculoviruses are one of the most studies insect viruses both in basic virology research and in biotechnology applications. This insect viruses based baculovirus expression vector system (BEVS) is an important tool for molecular biology and widely used in insect cells. Development of a shuttle baculovirus expression vector which can function in both the mammalian cell as well as in insect cell would have a significant advantages. We had cooperated an internal ribosome entry site (IRES) in to the baculovirus genomes and generated a bicistronic baculoviruses expression vector that can express two genes simultaneously. In this project, we attempt to construct a baculovirus vector with an IRES based bicistronic expression vector which using Bac-to-Bac system. The Bac-toBac system bring more benefits than the traditional methods using homologous recombination in insect cells. Bac-to-Bac plasmids: pFastBac1-Polh-MCS-Lir-DsRed2 had been successfully generated. Such IRES-based Bac-to-Bac BEVS will facilitate rapid and efficient generation of recombinant baculoviruses: time saving expression bacmid, one-step purification and amplification, rapid and simultaneous isolation of multiple recombinant viruses as well as titer determination

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